Volume 07,Issue 01

Study the Effect of Glycerol on Shape, Size, and Growth Rate of Escherichia Coli

Authors

Seyedeh Mahboobeh Hosseini Zare, Seyedeh Masoumeh Hosseini Zare, Shalaleh Masoumi, Maryam Zare

Abstract
Carbohydrate is a decisive factor in the growth of bacteria. Glucose is the most common carbon source of bacterial growth. However, the role of glycerol on bacterial growth remained to be clear. In this paper, the effects of glycerol on HF19 and MC1061 growth was examined. The optical density and colony formation unit per milliliter of cultured media was measured. Moreover, the cell dry mass of the cultured media has been assessed. In order to directly study the effect of glycerol on the E. coli cell proliferation, hemocytometer was used. The strong correlation was found between optical density and CFU/ml in the all incubation period in the control groups. The data showed very weak correlation between optical density and CFU/ml in the glycerol-treated group after 24-hour incubation. The data also indicated that glycerol increased the optical density, colony formation unit and cell dry mass of the sample. Although the data indicated strong relation in the control groups, in all incubating period, in the glycerol-treated group, we observed strong relation only in 6-hour and 12-hour incubation period. The data suggests that in long time glycerol incubation, glycerol induced changes in shape and/or size of E. coli in both strains.

Keyword: E. Coli, Glycerol, Growth rate.

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